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. Author manuscript; available in PMC: 2010 Oct 23.
Published in final edited form as: J Mol Biol. 2009 Aug 14;393(2):435–447. doi: 10.1016/j.jmb.2009.08.021

Figure 6.

Figure 6

Reaction mechanism and inactive conformation

A. The double displacement reaction mechanism in human α-NAGAL. D156 acts as the nucleophile and D217 acts as the acid/base. The ligand is bent into a high energy 1S3 skew boat conformation during the reaction. B. Human α-NAGAL without a soaked ligand shows a different conformation from all of the previous structures in the family. The residues D156 and Y192 shift into conformations where the nucleophilic lone pair rotates into a catalytically inactive location.