Skip to main content
. Author manuscript; available in PMC: 2010 Oct 13.
Published in final edited form as: Mol Cancer Res. 2009 Oct 13;7(10):1595–1604. doi: 10.1158/1541-7786.MCR-09-0072

Figure 5. PGK1 expression in PCa cells regulates expression of an osteoblastic phenotype.

Figure 5

To determine if PGK1 induces an osteoblastic phenotype in PCa cells, PGK1 was over-expressed and knocked-down in PC3 and C4-2B cells. (A) Western blot analysis for Runx2 and PGK1 expression in the PCa cells (PC3Control, PC3PGK1, PC3SiControl, PC3SiPGK1, C4-2BControl, C4-2BPGK1, C4-2BSiControl, and C4-2BSiPGK1). Quantitative assessment of data from (B&C) PC3Control, PC3PGK1, PC3SiControl, and PC3SiPGK1 cells and (D&E) C4-2BControl, C4-2BPGK1, C4-2BSiControl, and C4-2BSiPGK1 cells. Runx2 positive cells from immunohistochemistry for Runx2 expressed by (F) PC3Control, PC3PGK1, PC3SiControl, and PC3SiPGK1 cells and (G) C4-2BControl, C4-2BPGK1, C4-2BSiControl, and C4-2BSiPGK1 cells using monoclonal antibodies. (H&I) Alkaline phosphatase activity in the cell lysates of the PCa cells was elevated by ELISA. Data are presented as mean ± standard deviation. Significant difference is from controls. These data demonstrated that PCa-derived PGK1 regulates their own expression of osteoblastic phenotypes.