Figure 4. Methylation and expression of genes hypomethylated in ASC in comparison with NTERA-2 cells.

A, Differences of expression between ASC and NTERA-2 (blue bars) and promoter methylation in ASC (black diamonds) and NTERA-2 (red squares) are presented for each gene. The differences of expression, according to Affymetrix data, are shown as fold change (FC) in log₂. The methylation data are average BeadArray values. B, Bisulfite sequencing of promoter regions of COL1A2, HOXA9 and SERPINE1 in NTERA-2 and ASC cells. Promoter schematic description (black) and the location of the CpGs (blue) examined are presented for each gene. The CpG dinucleotides (clear box: unmethylated, filled box: methylated) of five clones and the BeadArray methylation values±standard deviation are shown for each sample. C, Expression of COL1A2, HOXA9 and SERPINE1 from Affymetrix arrays (colored rhombuses) and quantitative Q-RT-PCR (colored bars).