Table 1.
Data collection and refinement statistics.
| Data | FC-HGa | FC-CDb | MN-DEUTc | NI-DEUTd |
| Space Group | P212121 | P212121 | P212121 | P212121 |
| Wavelength | 0.98 | 0.98 | 0.98 | 0.98 |
| Resolution Range (Ǻ) | 50.0-1.6 | 50.0-1.8 | 50.0-2.0 | 50.0-2.2 |
| Outer Shell | 1.66-1.6 | 1.86-1.8 | 2.07-2.0 | 2.49-2.2 |
| Unique Observations | 224,750 | 82,065 | 116,112 | 64,956 |
| Completeness (%) | 99.9(99.6)e | 99.9(99.7) | 99.8(98.4) | 99.6(96.8) |
| Rsym (%)f | 0.08(0.24) | 0.05(0.19) | 0.07(0.22) | 0.06(0.26) |
| I/σ | 34.2(4.5) | 37.5(6.2) | 32.8(5.6) | 23.2(3.0) |
| Model | FC-HG | FC-CD | MN-DEUT | NI-DEUT |
| Unit Cell (a, b, c) in Ǻ | 86,93,109 | 86,93,109 | 88,94,109 | 86,93,110 |
| Protein Atoms | 5,985g | 5,848 | 5,839 | 5,790 |
| Solvent Atoms | 799 | 393 | 534 | 336 |
| Resolution Limits (Ǻ) | 50.0-1.6 | 50.0-1.8 | 50.0-2.0 | 50.0-2.2 |
| Rcryst (%) | 20.4 | 21.3 | 16.9 | 21.5 |
| Rfree (%) | 24.2 | 25.1 | 20.1 | 24.9 |
| rmsd bonds (Ǻ) | 0.006 | 0.009 | 0.006 | 0.008 |
| rmsd angles (°) | 1.23 | 1.31 | 1.24 | 1.29 |
| average B factor (Ǻ2) | 24.7 | 27.3 | 19.7 | 36.6 |
Data for crystals obtained with wild-type human ferrochelatase treated with protoporphyrin IX and either Hga or Cdb.
Data for crystals obtained with the F110A variant of human ferrochelatase treated with deuteroporphyrin either Mnc or Nid.
Numbers in parentheses denote values for the outermost resolution shell.
Rsym = ∑hkl[∑I(|Ihkl,I - <Ihkl>|)]/∑hkl,I<Ihkl>, where Ihkl is the intensity of an individual measurement of the reflection with indices hkl and <Ihkl> is the mean intensity of that reflection.
Number includes atoms for residues in alternate conformations. Coordinates for the FC-HG, FC-CD, MN-DEUT, and NI-DEUT data have been deposited in the Protein Data Bank under the PDBID codes 3HCN, 3HCO, 3HCP, and 3HCR respectively.