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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Am J Reprod Immunol. 2009 Aug;62(2):96–111. doi: 10.1111/j.1600-0897.2009.00717.x

Figure 1. Expression of β2GPI by first trimester trophoblast cells.

Figure 1

A) Lysates of the first trimester trophoblast cells (HTR8) and, as a positive control, placental tissue, were analyzed for β2GPI expression by Western blot. The trophoblast cells were positive β2GPI.

B) First trimester trophoblast cells (HTR8) were assessed for ID2 and IIC5 binding by immunocytochemistry. Cell were incubated with ID2 or IIC5 (40µg/ml) followed by a peroxidase-conjugated horse anti-mouse antibody (magnification ×60). Note the strong positive immunoreactivity of the trophoblast cells for ID2 and IIC5, localized to both the cytoplasm and plasma membrane. Control cells (Neg; magnification ×40) showed no staining.