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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Am J Reprod Immunol. 2009 Aug;62(2):96–111. doi: 10.1111/j.1600-0897.2009.00717.x

Figure 6. Effects of a MyD88-DN on the modulation of trophoblast cytokine/chemokine production by anti-β2GPI Abs.

Figure 6

Trophoblast cells (HTR8) were transiently transfected to express the MyD88-DN. Following transfection with either the MyD88-DN or the vector control, trophoblast cells were treated with either no treatment (NT), ID2 (20µg/ml), or IIC5 (20µg/ml) for 48 hours after which cell-free supernatants were collected and analyzed by multiplex analysis. Barcharts show changes in the secretion of i) IL-8; ii) MCP-1; iii) GROα; and iv) IL-1β. *p<0.05 and **p<0.001 relative to the NT was determined using ANOVA. Significant differences between the MyD88-DN and vector control were determined by paired t-test (*p<0.05; **p<0.001). Data are a representative of at least three independent experiments.