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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Chem Res Toxicol. 2008 Dec;21(12):2424–2432. doi: 10.1021/tx800333u

Figure 1.

Figure 1

Mass spectrometric analysis of eicosanoids from extracted supernatants of human neutrophils primed with GM-CSF (1 nM, 30 min) and then stimulated with fMLP (100 nM, 10 min) at 37°C in (A and B) the absence and (C and D) the presence of 25 μM acrolein. The GM-CSF primed cells were treated with acrolein for 3 min before fMLP was added. Elution of the eicosanoids was detected in the negative ion mode using MRM transitions specific to each metabolite as described in the Methods. The Δ6-trans-LTB4 isomers peak includes both Δ6-trans-LTB4 and Δ6-trans-12-epi-LTB4.