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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Chem Res Toxicol. 2008 Dec;21(12):2424–2432. doi: 10.1021/tx800333u

Figure 2.

Figure 2

Dose response of acrolein (0-50 μM) on the synthesis of (A) 5-LO products (20-OHLTB4, Δ6-trans-LTB4 isomers, LTB4, and 5-HETE) and (B) COX products (PGE2 and TXB2) formed in human neutrophils (PMN) primed with GM-CSF (1 nM, 30 min) and stimulated with fMLP (100 nM, 10 min) at 37°C. The GM-CSF primed cells were treated with acrolein for 3 min before fMLP was added. The control neutrophils were not treated with GM-CSF or fMLP. The 5-LO metabolites were analyzed by mass spectrometry and absolute quantities were calculated using relative abundances of the specific MRM relative to the deuterium labeled internal standard. Values (ng/2×106 cells) are expressed as mean ± S.E. (n = 5; ** p<0.01 and *** p<0.001).