Skip to main content
. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Chem Res Toxicol. 2008 Dec;21(12):2424–2432. doi: 10.1021/tx800333u

Figure 6.

Figure 6

Dose response of acrolein (0-500 μM) on the synthesis of Δ6-trans-LTB4 isomers and 5-HETE formed in a human recombinant 5-LO enzymatic assay. Human recombinant 5-LO (0.7 units) was incubated with ATP (2 mM) and acrolein (0-500 μM) for 3 min or zileuton (0.5 μM) for 15 min. After the preincubation, AA (0.2 μM) and Ca2+ (2 mM) were added and allowed to react for 10 min. The 5-LO metabolites were analyzed by mass spectrometry and absolute quantities were calculated using relative abundances of the specific MRM relative to the deuterium labeled internal standard. Values are expressed as mean ± S.E. (n = 3; *** p<0.001).