. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: J Therm Anal Calorim. 2009 Oct 1;98(1):97–104. doi: 10.1007/s10973-009-0228-8

TABLE I.

Liposome formulations Used in this Study

Matrix Lipid	Formulation^*	Matrix Lipid (%)	Other Phospholipids			Calcein Encapsulation Efficiency^#	Liposome Stability%	Photo Polymerize effect^$
Matrix Lipid	Formulation^*	Matrix Lipid (%)	C22:1 PC	C23:0 PC	DC_8,9PC	Calcein Encapsulation Efficiency^#	Liposome Stability%	Photo Polymerize effect^$

Egg PC (1)	1a	96	none	none	none	high	good	No

	1b	86	none	none	10 (mol %)	high	good	No

DNPC (2)	2a	86	10 (mol %)	none	none	no entrapment	n.a.	No

	2c	46	none	none	50 (mol %)	no entrapment	n.a.	Yes

	2b	86	none	10 (mol %)	none	no entrapment	n.a.	No

DMPC (3)	3a	86	none	none	10 (mol %)	high	poor	Yes

	3b	66	none	none	30 (mol %)	high	poor	Yes

	3c	46	none	none	50 (mol %)	high	poor	Yes

	3d	96	none	none	none	no entrapment	n.a.	No

DPPC (4)	4a	96	none	none	none	high	good	No

	4b	91	none	none	5 (mol %)	high	good	Yes

	4c	86	none	none	10 (mol %)	high	good	Yes

	4d	81	none	none	15 (mol %)	high	good	Yes

	4e	76	none	none	20 (mol %)	high	good	Yes

	4f	71	none	none	25 (mol %)	high	moderate	Yes

	4g	66	none	none	30 (mol %)	low	Poor	Yes

	4h	61	none	none	40 (mol %)	low	poor	Yes

	4i	56	none	none	50 (mol %)	low	poor	Yes

	4j ^{^^}	72	none	none	10 (mol %)	no entrapment	n.a.	N.A.

	4k ^{^^}	62	none	none	20 (mol %)	no entrapment	n.a.	N.A.

All preparations contained 4 mol% DSPE-PEG2000 for future in vivo applications.

Calcein encapsulation efficiency was compared with Egg PC liposomes (1a/1b).

Formulations that retained more than 75% entrapped calcein upon storage at 4°C up to 10 days were classified as stable and were used for further studies.

Photo polymerizable effect was measured be Appearance of red color after UV treatment

^{^^}

16 mol% DNPC was added to the liposome formulation