5-LOX phosphorylation in the total homogenate from post-mortem PFC of control subjects and suicide victims. Upper panel (A) shows typical examples of Ser523-phosphorylated 5-LOX (p5-LOX), total 5-LOX (both at the expected size of ~78 kDa), and β-actin (~ 42 kDa) immunoreactive bands.In the total homogenate, 5-LOX content did not differ between two groups but the p5-LOX content was elevated in the suicide samples (B). Results are the mean ± S.E.M. (n = 11; **p<0.01 vs. corresponding controls). Open bars, control (C); closed bars, suicide (S).