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Comparison of kinetic parameters for wild-type CBS and various second sphere heme-pocket mutants
| aSpecific Activity | wild-type | H67A | R266K | R266M |
|---|---|---|---|---|
| +PLP, +AdoMet | 316 ± 10 | 316 ± 17 | 343 ± 21 | 234 ± 22 |
| −PLP, −AdoMet | 220 ± 20 | 139 ± 12 | 115 ± 7 | 41 ± 1.4 |
| KMSer, mM | 2.0 ± 0.3 | 5.4 ± 1.6 | 4.4 ± 1.7 | 5.5 ± 0.5 |
| KMHcy, mM | 5.0 ± 0.9 | 3.4 ± 1.0 | 1.3 ± 0.3 | 1.6 ± 0.3 |
Specific activity is expressed in units of μmol h−1 mg protein−1.
