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. Author manuscript; available in PMC: 2010 May 1.

Published in final edited form as: J Inorg Biochem. 2009 Jan 22;103(5):689–697. doi: 10.1016/j.jinorgbio.2009.01.009

Table 2.

Comparison of specific activities of wild-type CBS and the R266 mutants^a

	wild-type	R266K	R266M
− HgCl₂	352 ± 27	343 ± 21	234 ± 22
300 μM HgCl₂	6.1 ± 1.6	^bND	^bND
CO bound-CBS	33.8 ± 4.2	5.2 ± 1.0	8.2 ± 3.5

^a

Assays were performed in 50 mM Tris-HCl, pH 8.6 in the presence of PLP and AdoMet and specific activity is reported in units ofμmol h⁻¹ mg protein⁻¹.

^b

Not detected.