Fig. 1.

Altered interactions between bmh1 mutant strains and cultured murine macrophages. (a) Candida was incubated with J774A.1 cells. WT (i–iv) and K51E (v–viii) were incubated for 1.5 h (i, v), 5 h (ii, vi) or 24 h (iii, iv, vii, viii). To assess macrophage viability, viable cells were stained with calcein AM (green) and Candida was counterstained with Calcofluor White (blue) (iv, viii). (b) Macrophage viability after 24 h co-incubation with bmh1 mutant yeast cells was assessed as described in Methods. Data are presented as the mean±sd number of viable macrophages per field. *Significantly different (P<0.0001) from isogenic control. The difference between M125R and BMH is not significant (P<0.06). (c) Macrophage viability after 24 h co-incubation with pre-germinated isogenic control (BMH) and the K51 bmh1 mutants was assessed as described in Methods. The experiment was repeated three times and the results combined. Data are presented as the mean±sem number of viable macrophages per field. Macrophage survival after incubation with K51E is significantly different from that for BMH (P≤0.002). (d) BMH and pre-germinated K51E cells were co-incubated with J774A.1 cells for 24 h and viable macrophages stained as in (a).