Fig. 2.

Attachment to and ingestion by J774A.1 cells of bmh1 mutants is similar to that of the WT. (a) Candida was pre-labelled with Oregon green, incubated for 1.5 h under cell culture conditions, fixed, and counterstained with Calcofluor White. Ingested Candida are stained green, while external Candida appear blue-green. A representative micrograph of external labelled Candida can be seen in Supplementary Fig. S1. (b) The numbers of attached and ingested Candida were assessed per 100 macrophages in two separate wells. A representative graph is depicted.