Table 1.
Phenotypes of bmh1 mutants
In vitro filamentation phenotypes of bmh1 mutants have been previously published.
| Strain | Growth* | Filamentation induction | Chlamy-dospores¶ | Macrophage viability# | Virulence** (days) | |||
|---|---|---|---|---|---|---|---|---|
| M199/FBS† (mm) | Embedment matrix‡ | Liquid FBS (min)§ | Chlamy-dospore|| | |||||
| 6284 | 1.0 | 5–6 | 100 % | 30 | 50–75 % | Yes | 0–6 | 7 |
| BH1P1 | 1.2 | 3–5 | 20–40 %†† | 30 | 0 % | No | nd | 5.5 |
| BMH | 1.2 | 3–5 | 20–30 %†† | 30–60 | 0 % | No | 0–5 | 7 |
| K51E | 3.5 | 0–1 | 5–9 %†† | 60–120 | 30–50 % | No | 20–150 | >31 |
| K51R | 1.3 | 0–2 | 0 % | 30–60 | 0 % | No | 0–6 | 12 |
| L231S | 3.3 | 0–1 | 19 %†† | 60–120 | 80–100 % | No | 15–115 | >31 |
| M125R | 1.2 | 3–6 | 100 % | 30 | 40–60 % | Yes | 4–16 | 24.5 |
| R142C | 1.0 | 5–6 | 100 % | 30 | 40–75 % | Yes | 0–7 | 7 |
*Lag time was calculated as time in hours to enter exponential growth compared with the WT control strain at 30 °C. Once cells entered exponential growth, generation times did not differ significantly (90–130 min, depending on the experiment).
†Cells were plated on M199 or 10 % FBS solid media and length of filaments was assessed at 7 days. L231S and K51E were afilamentous on FBS media; K51R was only sparsely filamentous on FBS media.
‡Cells were mixed with molten YPS (yeast peptone, 2 % sucrose) and monitored for 6 days, and the percentage of filamenting colonies per 100 colonies was counted.
§Cells were incubated in 10 % FBS; the ability to filament was assessed by onset of filamentation (min). Extensions of K51E and L231S filaments were not as long as seen for other strains.
||Cells were grown on cornmeal agar under microaerophilic conditions for 9 days, and the number of filamentous colonies was assessed.
¶Cells were grown on cornmeal agar under microaerophilic conditions and monitored for production or no production of chlamydospores.
#Strains were incubated with macrophages for 24 h, and macrophage viability was assessed as the mean number of macrophages per field (×20); nd, not determined.
**Virulence was assessed in the murine disseminated mouse model as described above and presented as median survival.
††Colonies were heterogeneous and smaller in size than those of the WT.