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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Cell Signal. 2009 Jul 5;21(11):1620–1625. doi: 10.1016/j.cellsig.2009.06.008

Fig. 1. TNF and IL-1β stimulate endogenous MLK3 kinase activity.

Fig. 1

A) SKOV3 cells were treated with 50 ng/ml TNF for indicated time periods and whole cell lysates were subjected to SDS-PAGE and Western blotting with a phospho-specific-MLK3 (p-MLK3), β-Actin and total MLK3 antibodies. B) HT-29 cells were treated with 50 ng/ml TNF for the indicated time periods and cell extracts were analyzed by Western blotting as described inA). C) SKOV3 cells were treated with IL-1β for the indicated time periods and cell extracts were analyzed by Western blotting as described in A).