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. Author manuscript; available in PMC: 2010 Sep 18.
Published in final edited form as: Cell. 2009 Sep 10;138(6):1236–1246. doi: 10.1016/j.cell.2009.06.043

Figure 2.

Figure 2

FRH regulates the frq RNA decay by the exosome. (A) Northern blot analysis showing the decay of frq RNA after the addition of thiolutin. Due to high levels of frq RNA after down-regulation of FRH, a shorter exposure of the Northern blots for the QA treated samples was used. The densitometric analysis of the results from three independent experiments are shown in the bottom. (B) Northern blot analysis showing the results of the RNAse H assay for frq RNA. RNA samples from the wild-type and dsfrh strains were used. Due to high levels of frq RNA in the dsfrh samples, less RNA for the dsfrh samples was applied to bring frq signals to comparable levels for both sets of the samples. (C) Western blot analysis showing the results of immunoprecipitation assay using the FRH antibody. Immunoprecipitation using the FRH pre-immune (PI) serum was used as the control. Myc-RRP44, Myc-RRP6 and Myc-QIP (the negative control) strains were used.