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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Chem Res Toxicol. 2008 Dec;21(12):2313–2323. doi: 10.1021/tx8002998

Figure 2.

Figure 2

Dependence of inhibition of P450 1B1-supported EROD activity by B[a]P on incubation time. P450 1B1, at concentrations of 3.3 nM (parts A and D), 13 nM (parts B and E), and 50 nM (parts C and F) was used without B[a]P (closed circles in parts A, B, and C, ●), 25 nM B[a]P (open circles, ○), 50 nM B[a]P (open triangles, Δ), and 100 nM B[a]P (open squares, □). Reactions were done at 37 °C. Formation of resorufin was monitored as a function of incubation time in parts A, B, and C, and the effects of B[a]P were determined as log10 (% activity remaining) in parts D, E, and F.