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Intracellular calcium increase on exposure to CXCR3 ligands in K7M2 and SAOS-LM7 cells. K7M2 (a) and SAOS-LM7 (b) osteosarcoma cells were loaded with Fluo-4 AM and exposed to 100 ng/mL CXCL9 (▴) and 50 ng/mL CXCL10 (∎) or CXCL11 (◻). Calcimycin (●) served as positive control agonist, whereas the addition of base medium alone (○) served as the negative control. Calcium mobilization is defined as increased fluorescent intensity of cells.
