. Author manuscript; available in PMC: 2009 Nov 3.

Published in final edited form as: Chem Res Toxicol. 2008 Aug 26;21(10):1956–1963. doi: 10.1021/tx800138v

Table 2. Effect of Inactivation by 17EE on the Ability of CYPs 2B1 and 2B6 to Metabolize and Bind Substrates^a.

	% Remaining compared to Non-Inactivated Controls

CYP	Activity	n-Octylamine 431 nm- 410 nm	Benzphetamine 385 nm- 418 nm
2B1	34	45	39
2B6	21	19	34

CYPs 2B1 or 2B6 were reconstituted with reductase and incubated with 17EE in the presence or absence of NADPH as described in Experimental Procedures. Aliquots were removed and assayed for residual 7EFC activity and for spectral binding changes upon addition of the substrates indicated.

Table 2. Effect of Inactivation by 17EE on the Ability of CYPs 2B1 and 2B6 to Metabolize and Bind Substratesa.

Table 2. Effect of Inactivation by 17EE on the Ability of CYPs 2B1 and 2B6 to Metabolize and Bind Substrates^a.