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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Biochem Pharmacol. 2009 May 27;78(7):795–802. doi: 10.1016/j.bcp.2009.05.022

Figure 3.

Figure 3

Time Courses for the Onset and Recovery of ABT-089 Induced Desensitization. The time course for the onset of desensitization by exposure to 1 μM ABT-089 is shown in panel A. Thalamic synaptosomes were exposed to 1 μM ABT-089 for the indicated times before stimulation with 10 μM nicotine for 1 min. Each point represents the mean ±SEM of three separate experiments. The curve is the least squares curve fit of the data to an exponential decay with residual activity. The time course for recovery from desensitization elicited by exposure to 1 μM ABT-089 is shown in Panel B. Synaptosomes were perfused 1 μM ABT-089 for 10 min after which they were perfused with drug free buffer before stimulation with 10 μM nicotine for 1 min. Each point represents the mean±SEM of data from three separate experiments. The curve represents the exponential recovery of activity starting at a non-zero point. The values for the rate constants calculated from the curve fits are listed in the figure.