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. Author manuscript; available in PMC: 2009 Nov 3.
Published in final edited form as: Nat Neurosci. 2008 Sep;11(9):995–997. doi: 10.1038/nn.2178

Figure 2.

Figure 2

Many BrdU-labeled cells in the AVPV are mature neurons, not astrocytes. Many BrdU-labeled cells in Me are astrocytes; some are neurons. a: Confocal images of cells in male and female rat AVPV. Sections were processed for double-label BrdU (green) and the mature neuron marker NeuN (red); colocalization is yellow. b: Orthogonal views of confocal images verify colocalization of BrdU and NeuN in the female AVPV of a neuron that was born on P30-32. c: Confocal image of cells in rat Me. Section was processed for triple-label BrdU (green), NeuN (blue), and the astrocytic glial marker GFAP (red). Orthogonal views verify colocalization of BrdU, which is in the nucleus, and GFAP, which is in astrocytic processes. Scale bars: 10 μm.