Table II. Sensitivity of Cell Lines to K252a.
A panel of cancer cell lines was analyzed for Trk expression via immunoblot, neurotrophin expression by ELISA, and cell death after exposure to 100nM K252a or 10μM AG879 as indicated. Suspension cells were analyzed for apoptosis by flow cytomety using Annexin V and propidium iodide staining. Apoptosis in adherent cells was determined by MTT assay and ApoPercentage Assay. ‘+’ indicates low level of Trk or neurotrophin expression, ‘++’ indicates intermediate expression levels and ‘+++’ indicates high expression levels. NA indicates assay not performed because it was not appropriate for designated cell type. ND indicated not determined due to variability in results. +( indicates sensitivity to K252a and AG879.
| Cell Line | Trk Expression | NGF | BDNF | MTT | ApoPercentage | Annexin V |
|---|---|---|---|---|---|---|
| 293T | ++ | + | - | - | +ψ | NA |
| CHME5 | + | +++ | - | +ψ | ND | NA |
| MCF-7 | ++ | + | - | +ψ | +ψ | NA |
| MDA MB231 | + | - | + | - | - | NA |
| THP-1 | +++ | - | + | NA | NA | +ψ |
| Ramos | ++ | - | - | NA | NA | - |
| Raji | + | - | - | NA | NA | - |
| Daudi | ++ | - | - | NA | NA | - |
| RPMI-8226 | + | - | - | NA | NA | - |