Figure 3. Cytokine/chemokine mRNA expression in the lungs of vaccinated mice is rapidly up-regulated following i.t. challenge with F. tularensis LVS.
Groups of control (solid bar) and vaccinated (open bar) mice were infected i.t. with 6 LD50 F. tularensis LVS. The lungs were dissected at 4 hours and 3 days post-infection; cytokine and chemokine mRNA expression was quantified by real-time RT-PCR. Data are the means ± SD derived from 4 mice treated comparably. A second experiment yielded similar results. *Vaccinated group is significantly different from control: P<0.05.