Table 2.
Real-time RT-PCR Primers
| Gene | GenBank accession |
Orientation | Primer sequence |
Amplicon size |
|---|---|---|---|---|
| Interleukin-1β | NM_008361 | forward | TCACAGCAGCACATCAACAA | 112 |
| reverse | TGTCCTCATCCTGGAAGGT | |||
| Interleukin-2 | NM_008366 | forward | CCCACTTCAAGCTCCACTTC | 155 |
| reverse | ATCCTGGGGAGTTTCAGGTT | |||
| Interleukin-4 | NM_021283 | forward | CCTCACAGCAACGAAGAACA | 155 |
| reverse | ATCGAAAAGCCCGAAAGAGT | |||
| Interleukin-6 | NM_031168 | forward | AGTTGCCTTCTTGGGACTGA | 103 |
| reverse | CAGGTCTGTTGGGAGTGG | |||
| Interleukin-10 | NM_010548 | forward | CCAAGCCTTATCGGAAATGA | 162 |
| reverse | TTCACAGGGGAGAAATCG | |||
| Interleukin-12 (p40) | NM_008352 | forward | GGAAGCACGGCAGCAGAATA | 180 |
| reverse | AACTTGAGGGAGAAGTAGGAATG | |||
| IFN-γ | NM_008337 | forward | ACTGGCAAAAGGATGGTGAC | 211 |
| reverse | ACCTGTGGGTTGTTGACCTC | |||
| TNF-α | NM_013693 | forward | GTTCTGCAAAGGGAGAGTGG | 106 |
| reverse | GCACCTCAGGGAAGAGTCT | |||
| MIP-1α (Ccl3) | NM_011337 | forward | ACCATGACACTCTGCAACCA | 202 |
| reverse | CCCAGGTCTCTTTGGAGTCA | |||
| MCP-1 (Ccl2) | NM_011333 | forward | CCCACTCACCTGCTGCTACT | 164 |
| reverse | TCTGGACCCATTCCTTCTTG | |||
| KC (Cxcl1) | NM_008176 | forward | GCTGGGATTCACCTCAAGAA | 169 |
| reverse | TGGGGACACCTTTTAGCATC | |||
| 18S ribosomal | XR_000144 | forward | AATGGTGCTACCGGTCATTCC | 192 |
| RNA | reverse | ACCTCTCTTACCCGCTCTC |