TABLE 2.

Effects of various esterase inhibitors on the conversion of CS-8958 to R-125489 in mouse trachea and lung S9 fractions^a

Condition	CS-8958 hydrolase activity (% of control) in S9 fractionsb
	Trachea	Lung
Control	100.0	100.0
DFP	44.1	13.6
Eserine	—	31.9
BNPP	—	21.0
DTNB	—	42.0
EDTA	—	130.7
PCMB	—	106.5

^aVarious esterase inhibitors (final concentration, 1 mM) were each added to the trachea and lung S9 fractions 10 min before the addition of [¹⁴C]CS-8958 (final concentration, 10 μM) in order to examine their effects on its hydrolysis at 37°C. The experiment was performed in the presence of 1% dimethyl sulfoxide. The control values for the hydrolase activities were 4.51 and 1.01 pmol/min/mg in lung and trachea S9 fractions, respectively.

^bValues are means for duplicate experiments. —, not tested due to the limitations of the matrix.