TABLE 1.
Strains and plasmids used in the study
| Strain or plasmid | Description | Reference |
|---|---|---|
| E. coli strains | ||
| DH10B | pMCT-pvdQ | 16 |
| S17-1 | galU galK rpsL(Strr) endA1 nupG thi pro hsdR hsdM+recA (RP4-2Tc::Mu Km::Tn7) λpir | 8 |
| P. aeruginosa strains | ||
| PAO1 | Wild type, Marseille strain | |
| PAO1ΔpvdQ | ΔpvdQ chromosomal deletion mutant derived from PAO1 | This study |
| PAO1(pMEpvdQ) | pvdQ overexpressing strain derived from PAO1 | 16 |
| Plasmids | ||
| pME6032 | lacIQ-Ptac expression vector; pVS1-p15A shuttle vector TetR | 7 |
| pME6032-pvdQ | pvdQ in pME6032 | 16 |
| pSB1075 | lasR lasI′ (P. aeruginosa PAO1)::luxCDABE (P. luminescens [ATCC 29999]) fusion in pUC18 Apr, with acyl-HSL biosensor producing bioluminescence | 24 |