TABLE 2.
PCR/RT-PCR primers used in this study
| Genea | Sequencesb | Tm (°C)b | Product size (bp)b |
|---|---|---|---|
| ftsZ | Forward, 5′-ATGGAACTTACCAATGACGCG-3′ | 57 | 100 |
| Reverse, 5′-TCAACACCTTCAATGCGCTC-3′ | 56 | ||
| blaTEM-1 | Forward, 5′-GCATCTTACGGATGGCATGA-3′ | 56 | 100 |
| Reverse, 5′-GTCCTCCGATCGTTGTCAGAA-3′ | 59 | ||
| tetA(C) | Forward, 5′-TCTAACAATGCGCTCATCGTCATCC-3′ | 61 | 109 |
| Reverse, 5′-GGAATGGACGATATCCCGCA-3′ | 57 | ||
| rop | Forward, 5′-CAGGAAAAAACCGCCCTTAACATG-3′ | 59 | 101 |
| Reverse, 5′-ATGTCTGCCTGTTCATCCGC-3′ | 54 | ||
| cpsE | Forward, 5′-CTATTCTCGACGCCATCAACG-3′ | 59 | 104 |
| Reverse, 5′-CCTGCAAAGAAATGCGCTCT-3′ | 59 |
a
Primers were used for determinations of pBR322 plasmid copy number and mRNA gene expression levels. CpsE, colonic acids biosysthesis gene; blaTEM-1, TEM-1 β-lactamase gene; tetA(C), tetracycline efflux pump gene; rop, pBR322 plasmid stabilization gene; ftsZ, cell division FtsZ protein (E. coli essential gene).
b
Primers were designed by using Primer Express software (Applied Biosystems). The primer conditions were determined according to the dye manufacturer.