TABLE 1.
Antimicrobial data for E. coli N08-1503 and the transformants harboring pT168a
| E. coli strain | Disk diameter (mm) |
MIC (μg/ml) |
|||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Broth microdilution |
ESBL Etest |
Etest |
|||||||||||||||||||||
| Cefotaximeb | Cefotaxime-CLAc | Ceftazidimeb | Ceftazidime-CLAc | Cefpodoximeb | Cefpodoxime-CLA | Cefotaximeb | Cefotaxime-CLA | Ceftazidimeb | Ceftazidime-CLAc | Cefpodoximeb | Cefpodoxime-CLA | Cefepime | Cefepime-CLA | Cefotaxime | Cefotaxime-CLAd | Ceftazidime | Ceftazidime-CLAd | Cefepime | Cefepime-CLAe | Amoxicillin-CLA | Pipercillin-TZBg | Cefoxitin | |
| N08-1503 (pT168) | 23 (pos) | 24 (neg) | 8 (pos) | 11 (neg) | 20 (neg) | 15 | ≤0.25 (neg) | ≤0.12 | 4 (pos) | 2 (neg) | 8 (pos) | 0.5 | 8 | ≤0.125 | 0.25 | 0.094 (neg) | 3 | 0.75 (neg) | 2 | 0.094 (pos) | 32 | >256 | 12 |
| DH10B (pT168) | 26 (pos) | 30 (neg) | 8 (pos) | 10 (neg) | 20 (neg) | 16 | ≤0.25 (neg) | ≤0.12 | 4 (pos) | 1 (neg) | 4 (neg) | 0.25 | 4 | ≤0.125 | 0.25 | 0.094 (neg) | 3 | 1 (neg) | 2 | 0.094 (pos) | 64 | >256 | 6 |
| DH10B | 29 (neg) | 33 | 16 (pos) | 18 (neg) | 27 (neg) | 21 | ≤0.25 (neg) | ≤0.12 | ≤0.25 (neg) | 0.25 | 1 (neg) | ≤0.125 | ≤0.25 | ≤0.125 | <0.25 | 0.094 (neg) | 1 | 0.38 (neg) | <0.25 | <0.25 (neg) | 3 | 1.5 | 8 |
CLA, clavulanic acid; TZB, tazobactam; pos, positive; neg, negative.
Data in parentheses are the interpretations for the screen for ESBL according to CLSI document M100-S19 (2).
Data in parentheses are the interpretation for confirmation of ESBL according to CLSI document M100-S19 (2).
Data in parentheses are the interpretation for confirmation of ESBL according to the manufacturer's instructions in document 75001448-MH0383 (Etest for detection of ESBL; AB bioMérieux, Solna, Sweden). The overall interpretation of the phenotype is non-ESBL producer as both strips were negative.
Data in parentheses are the interpretation for confirmation of ESBL according to the manufacturer's instructions in document 75002213-MH0344 (Etest for detection of ESBL; AB bioMérieux, Solna, Sweden). The overall interpretation of the phenotype is ESBL producer as one strip was positive.