TABLE 1.
Primers used for amplification of resistance genes and replicon typing
| Primer | Target gene/site | Sequence (5′ to 3′) | Annealing temp (°C) | Reference |
|---|---|---|---|---|
| TEMF | blaTEM | ATGAGTATTCAACATTTCCGTG | 55 | 12 |
| TEMR | TTACCAATGCTTAATCAGTGAG | |||
| SHV S1 | blaSHV | ATTTGTCGCTTCTTTACTCGC | 55 | 12 |
| SHV S2 | TTTATGGCGTTACCTTTGACC | |||
| CTX-M/F | blaCTX-M | TTTGCGATGTGCAGTACCAGTAA | 51 | 14 |
| CTX-M/R | CGATATCGTTGGTGGTGCCATA | |||
| CTX-M-2 group F | blaCTX-M-2 | AAATGTGCTGCTCCTTTCGTGAGC | 60 | 12 |
| CTX-M-2 group R | AGGGTTCGTTGCAAGACAAGACTG | |||
| CTX-M-9 group F | blaCTX-M-9 | GTGACAAAGAGAGTGCAACGC | 60 | 34 |
| CTX-M-9 group R | ATGATTCTCGCCGCTGAAGCC | |||
| CTX-M-15-SF | blaCTX-M-15 | CACACGTGGAATTTAGGGACT | 55 | 26 |
| CTX-M-15-SR | GCCGTCTAAGGCGATAAACA | |||
| CMY-F | blaCMY | CCGGACACCTTTTTGCTTTT | 60 | 35 |
| CMY-R | TATCCTGGGCCTCATCGTCAGTTA | |||
| OXA-1 F | blaOXA-1 | TTTTCTGTTTGGGTTTT | 52 | 2 |
| OXA-2 R | TTTCTTGGCTTTTGTGCTTG | |||
| aac(6′)-1b f | aac(6′)-Ib | TTGCGATGCTCTATGAGTGGCTA | 55 | 28 |
| aac(6′)-1b r | CTCGAATGCCTGGCGTGTTT | |||
| rfb.1bis | O typing for O25 | ATACCGACGACGCCGATCTG | 60 | 9 |
| rfb25.r | TGCTATTCATTATGCGCAGC |