TABLE 2.
Bacterials strains and plasmidsa
| Strain or plasmid | Genotype or characteristic(s) | Source or reference |
|---|---|---|
| E. coli strains | ||
| Wild-type AG100 | argE3 thi-1 rpsL xyl mtl supE44 λ lysogen | 11 |
| lon mutant | AG100 lon3::IS186 | 23 |
| lon ycgE mutant | AG100 lon3::IS186 ycgE::Tn5 | This study |
| ycgE mutant | AG100 ycgE::Tn5 by P1 transduction of AG100 × lon ycgE | This study |
| marR mutant | AG100 marR mutant | 24 |
| ΔmarCOAB::kan mutant | AG100 in which the marCORAB divergon is deleted | 15 |
| Plasmids | ||
| pACT7 | T7 RNA polymerase regulated by the lacUV5; ori p15A; Kanr | 18 |
| pACT7Sp | pACT7 Spr; this plasmid is used in conjunction with pET21b | This study |
| pET21b | Expression cloning vector; Ampr; the transcription of the cloned gene is driven by the T7 RNA polymerase and controlled by the LacI repressor | Novagen |
| pET21b-ycgE | pET21b in which ycgE was cloned using the NdeI and XhoI restriction sites | This study |
| pET21b-ompF | pET21b in which ompF was cloned using the NdeI and XhoI restriction sites | This study |
| pMPM | Expression cloning vector; Tetr; ori p15A; the araBAD promoter initiates the transcription of the cloned gene | 17 |
| pMPM-ompF | pMPM in which ompF was cloned using the EcoRI and XhoI restriction sites | This study |
| pKD46 | Red helper plasmid carrying the Red recombinase from the phage λ; Ampr; ori R101 | 9 |
| pKD3 | Plasmid carrying an FRT-flanked chloramphenicol resistance (cat) gene; Cmr; ori Rγ | 9 |
| pCP20 | Flp helper plasmid carrying gene encoding the yeast Flp recombinase; thermal induction of Flp; Ampr Cmr; λpR Repts | 5 |
a
Amp, ampicillin; Cm, chloramphenicol; Sp, spectinomycin; Tet, tetracycline.