TABLE 3.
MIC determined by Etests on LB medium at 37°C and 26°Ca
| Strain | MIC (μg/ml) at 37°C/26°C |
||||||
|---|---|---|---|---|---|---|---|
| FX | AM | CE | Cm | TC | DC | NA | |
| Wild-type AG100 | 1.5/0.75 | 1/0.75 | 2/1 | 4/4 | 1/0.5 | 1/0.5 | 4/4 |
| lon mutant | 1.7/0.92 | 1/0.83 | 2/1.2 | 4/4 | 1/0.5 | 1.17/0.75 | 4/3.7 |
| ycgE mutant | 1.7/0.92 | 1/0.75 | 2.3/1.3 | 4/4 | 1/0.5 | 1.17/0.83 | 4/3.7 |
| lon ycgE mutant | 4/4 | 1.7/1.8 | 4.7/6 | 9.3/12 | 2/2 | 2/2 | 4/3.3 |
| marR mutant | 6/7.3 | 3/4 | 9.3/10.7 | 21/24 | 4/6 | 3.3/3.7 | 10.7/6.7 |
| ΔompF mutant | 3/3 | 1.83/1.5 | 4.7/4.7 | 6.7/6 | 1.83/2 | 2/2 | 4/3.3 |
| lon ycgE ΔompF mutant | 3/4.7 | 2.2/2.3 | 4.7/6.7 | 10.7/13.3 | 2/2 | 2/2 | 4/3.3 |
| lon ycgE mutant carrying pMPM | 6 | 2.3 | 5.3 | ND | 3 | ||
| lon ycgE mutant carrying pMPM-ompF | 1 | 1.5 | 2 | ND | 3 | ||
| lon ycgE mutant carrying pET21b and pACT7SP | ND | ND | ND | 2 | 3 | ||
| lon ycgE mutant carrying pET21b-ompF and pACT7Sp | ND | ND | ND | 0.75 | 3 | ||
a
FX, cefoxitin; AM, ampicillin; CE, cephalothin; Cm, chloramphenicol; TC, tetracycline; DC, doxycycline; NA, nalidixic acid; ND, not able to be determined because of intrinsic resistance of the host. Numbers represent an averages of at least four experimental measurements. The standard errors of the means were <20% for all experiments. For the mutants carrying plasmids, MICs were determined at 26°C.