TABLE 1.
HIV-1 production by 4′-Ed4T mutant variants in CEM cells
| Virus | HIV-1 RNA (copy no. [103]/ml) |
|
|---|---|---|
| Day 2 | Day 4 | |
| pNL4-3 (wild type) | 61.4 ± 0.8b | 159.2 ± 11.8 |
| P119S | 50.3 ± 4.7 | 140.9 ± 22.8 |
| T165A | 52.2 ± 1.1 | 118.2 ± 2.5 |
| M184V | 52.3 ± 9.1 | 138.4 ± 13.0 |
| P119S/T165A | 76.2 ± 1.2 | 147.0 ± 16.5 |
| P119S/M184V | 49.3 ± 13.2 | 133.4 ± 15.3 |
| T165A/M184V | 47.0 ± 7.9 | 134.8 ± 21.2 |
| P119S/T165A/M184V | 25.3 ± 6.8 | 119.5 ± 16.1 |
| pTKDa | 41.7 ± 2.0 | 127.8 ± 21.6 |
a
A chimeric NL4-3 clone with the full RT region replaced by the corresponding RT sequence in the IIIB background carrying the P119S/T165A/M184V mutations, which was isolated by Nitanda et al. (32).
b
The data represent the means ± standard deviations for two separate experiments.