TABLE 3.

Steady-state kinetic parameters for inhibition of wild-type RT and drug-resistant mutants by 4′-Ed4TTP and d4TTP

Enzyme	dTTP			4′-Ed4TTP		d4TTP
	Km(dTTP) (μM)	kcat (s−1)	kcat/Km(dTTP) (μM−1 s−1)	Ki (μM)	Ki(4′-Ed4TTP)/Km(dTTP)	Ki (μM)	Ki(d4TTP)/Km(dTTP)
Wild-type RT	0.12 ± 0.03a	3.2 × 10−3 ± 1.4 × 10−4	2.7 × 10−2	0.054 ± 0.016	0.45	0.63 ± 0.05	5.3
P119S	0.13 ± 0.03	3.3 × 10−3 ± 1.7 × 10−4	2.5 × 10−2	0.11 ± 0.013	0.85	0.64 ± 0.01	2.1
T165A	0.10 ± 0.008	2.7 × 10−3 ± 3.5 × 10−5	2.7 × 10−2	0.08 ± 0.02	0.8	0.21 ± 0.03	2.1
M184V	0.12 ± 0.04	3.1 × 10−3 ± 2.6 × 10−4	2.6 × 10−2	0.21 ± 0.03	1.7	0.79 ± 0.17	6.6
P119S/T165A	0.07 ± 0.01	3.6 × 10−3 ± 1.2 × 10−4	5.1 × 10−2	0.024 ± 0.003	0.34	0.37 ± 0.02	5.3
P119S/M184V	0.18 ± 0.03	4.0 × 10−3 ± 3.6 × 10−5	2.2 × 10−2	0.64 ± 0.18	3.6	0.48 ± 0.02	2.7
T165A/M184V	0.11 ± 0.02	1.4 × 10−3 ± 4.8 × 10−5	1.3 × 10−2	0.66 ± 0.12	6	0.3 ± 0.06	2.7
P119S/T165A/M184V	0.15 ± 0.03	9.0 × 10−4 ± 3.6 × 10−5	6.0 × 10−3	0.73 ± 0.10	4.9	1.05 ± 0.2	7

^aThe data represent the means ± standard deviations for three separate experiments.