FIG. 1.
Nucleotide sequence of a 3,630-bp BamHI fragment of recombinant plasmid p7037B1 containing the blaVEB-1a gene. The deduced amino acid sequence is designated in single-letter code below the nucleotide sequence. The transcription orientations of the ORFs are indicated by horizontal arrows (orf is the unknown gene, and xre is the gene encoding a putative transcriptional regulator). The right-end boundary of ISCR2, defined as oriIS, is shaded. The putative promoter sequences of the blaVEB-1a gene, consisting of the −35 and −10 regions, are boxed. The ΔtnpA truncated transposase gene of ISCR2 is indicated. The stars indicate stop codons. The vertical arrows indicate the boundaries of the blaVEB-1a gene cassette as found in class 1 integrons (with the core site sequence in bold) and also the location where ΔISCR2 is identified. The BamHI sites of the cloned fragment are underlined.