TABLE 1.
IC50s of recombinant RTs for NNRTIs by RDDP filter-based assay and DDDP gel-based assay
| Assay and RT mutation | Drug susceptibility (IC50)a |
|||
|---|---|---|---|---|
| ETR (nM) | DAP (nM) | EFV (nM) | NVP (μM) | |
| RDDP | ||||
| WT | 175 ± 32 | 370 ± 32 | 93 ± 14 | 11 ± 2 |
| G190A | 191 ± 25 | 517 ± 49 | 112 ± 12 | >1,000 |
| Y181C | 257 ± 35 | 570 ± 69 | 126 ± 23 | >1,000 |
| G190A-181C | 162 ± 10 | 404 ± 31 | 100 ± 18 | >1,000 |
| DDDP | ||||
| WT | 97 ± 13 | 322 ± 45 | 18 ± 3 | 0.7 ± 0.1 |
| G190A | 129 ± 22 | 316 ± 41 | 216 ± 31 | >67 |
| Y181C | 107 ± 16 | 430 ± 52 | 36 ± 5 | >67 |
| 190A-81C | 279 ± 25 | 483 ± 33 | 380 ± 43 | >67 |
a
Data represent the means ± standard deviations of three separate determinations.