TABLE 2.
Susceptibilities of viruses harboring G190A to NNRTIs in cell-based phenotypic assays
| Isolate (mutation) | Drug susceptibility (EC50)a |
|||
|---|---|---|---|---|
| NVP | EFV | ETR | DAP | |
| 5269 (WT) | 0.007 | 0.00025 | 0.00090 | 0.0003 |
| 5331 (WT) | 0.010 | 0.00044 | 0.00251 | 0.0040 |
| 5512 (WT) | 0.020 | 0.00019 | 0.00226 | 0.0105 |
| Mean | 0.012 ± 0.003 | 0.00029 ± 0.00008 | 0.00189 ± 0.00050 | 0.0049 ± 0.0029 |
| 8116 (A98S, G190A) | 0.852 (71) | 0.00103 (3.6) | 0.00153 (0.8) | 0.0001 (0.02) |
| 8117 (A98S, G190A) | 0.703 (58) | 0.00030 (1.0) | 0.00065 (1.3) | 0.0002 (0.04) |
| 9225 (A98S, G190A) | 0.997 (83) | 0.00085 (2.9) | 0.00017 (0.9) | 0.00035 (0.07) |
| Mean | 0.850 ± 0.084 (70) | 0.00072 ± 0.00022 (2.5) | 0.00078 ± 0.00039 (0.4) | 0.0002 ± 0.0001 (0.04) |
a
The drug susceptibilities of WT viruses and resistant viruses harboring the A98S and G190A mutations were determined in two to three separate experiments. The overall susceptibilities of the WT RT and the RT with the G190A mutation are expressed as the means ± standard errors of the means. The values in parentheses are the fold resistance.