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SDS-PAGE and Western blot analysis of gp96, N336, P24, and p24-N336. (A) Purified p24 (lane 1) and p24-N336 (lane 2) were analyzed on a 12% SDS-PAGE gel and were immunoblotted with an anti-p24 monoclonal antibody, respectively (lanes 4 and 3). (B) Purified gp96 (lane 1), N336 (lane 2), and p24-N336 (lane 3) were analyzed on a 12% SDS-PAGE gel and immunoblotted with an anti-gp96 monoclonal antibody (lanes 6, 5, and 4).
