Antibody response to recombinant VlsE and Borrelia lysate antigen on day 56 postinoculation in a Western blot. M, positive control marker for protein bands; N, negative murine serum. Number 1, stripes a to c: mice inoculated with B. burgdorferi sensu stricto N40 (B02, B12, and B19). Number 2, stripes a to c: mice inoculated with B. garinii PBi (G07, G13, and G18). Number 3, stripes a to c: mice inoculated with B. afzelii PKo (A08, A12, and A17). Number 4, stripes a to c: mice inoculated with B. afzelii Slovakia (AS07, AS14, and AS15). Number 5, stripes a to c: mice inoculated with B. spielmanii A14S (S09, S22, and S25). Number 6, stripes a to c: mice inoculated with B. lusitaniae (L04, L01, and L12). Number 7, stripes a to c: mice inoculated with B. valaisiana VS116 (VS02, VS03, and VS05). Number 8, stripes a to c: mice inoculated with B. valaisiana Rio6 (VR03, VR05, and VR08).