FIG. 3.

(A) Western blot profiles of B. procyonis larval ES antigenic components recognized by human serum samples negative (EIA titers of 1:2 and 1:8) for Toxocara spp. Toxocara-negative sera show no or very mild reactivity with some of the antigenic components of B. procyonis larval ES antigens and none with the 30- to 45-kDa antigens (Box). Lane S, protein standard; lane P, serum from B. procyonis-infected baboon as a positive control; lane N, human negative control serum; lanes 1 to 15, Toxocara larva migrans-negative human patient sera. Five-microgram portions of antigen per lane were used. All sera were used at a 1:1,000 dilution. (B) Western blot profiles of B. procyonis larval ES antigenic components recognized by human serum samples specifically positive (EIA titer of >1:256) for Toxocara spp. Toxocara-specific sera of high or unknown (Txc 1 to 7) EIA titers recognize different antigenic components of B. procyonis larval ES antigens, but not the 30- to 45-kDa antigenic components (except for lane 7*) (box). Lane S, protein standard; lane P, serum from B. procyonis-infected baboon as a positive control; lane N, human negative control serum; lanes 1 to 22, Toxocara larva migrans-positive human patient sera. Five-microgram portions of antigen per lane were used. All sera were used at a 1:1,000 dilution. (Suspected dual infection with Toxocara spp. and B. procyonis is indicated by an asterisk.)