FIG. 3.

Parasite burden in the infected footpad and draining lymph node of the four mice strains vaccinated with LACK+CpG and LmPDI+CpG antigens and then challenged with L. major parasites. The parasite burden was determined by limiting-dilution assay at week 10 (PWK, C57BL/6, and BALB/c mice) or week 20 (MAI mice) postchallenge in vaccinated (with LACK+CpG [▨] or LmPDI+CpG [▩]) and control groups (PBS only [▪] or CpG only [□]). The results are expressed as the means ± the standard deviations of the log₁₀ dilutions of infected footpads (A) or draining popliteal lymph nodes (B) positive for L. major promastigotes. The results represent triplicates from five individuals in each group. The results correspond to those obtained from one representative experiment out of two performed in different PWK, BALB/c, and C57BL/6 mouse groups.