FIG. 1.

Cluster analysis of screening data. Heat map overview of log₁₀-transformed MFI data from the bead-based screening of 134 samples (115 sera) against 61 recombinant M. mycoides SC surface proteins and His₆ABP. Color intensity denotes signal intensity. Proteins against which large amounts of serum antibodies were detected in CBPP-positive sera, thus suggesting diagnostic importance, formed a cluster of nine proteins (top of vertical dendrogram; the cluster is highlighted in red). Horizontally, sera were separated into five groups. Clusters I, II, and V contained a majority of the CBPP-positive samples (red). Cluster III contained all but one hyperimmune serum (yellow), which displayed signals of low intensities, while cluster IV contained mostly CBPP-negative bovine sera (blue) as well as the positive control antiserum to M. mycoides SC PG1. All technical replicates clustered together, except for one specimen of a Swedish negative control serum.