FIG. 5.

Reproducibility of antigen cocktail ELISA. Two independent ELISA experiments with 15 sera in triplicate were performed by different persons 1 month apart to determine the reproducibility of the assay. The two bars for each sample represent the mean value for each experiment, and the dots represent the maximum and minimum values. All signals were normalized to the reference serum B001, resulting in low intra- and interassay variation (average CV, 3% and 8%, respectively). A percent positivity of 50% was suggested as the diagnostic cutoff, shown by a dashed line.