FIG. 1.

(A) pCMV-IgK-gE2gD-TM vector diagram (not to scale) containing the cytomegalovirus enhancer promoter (CMV prom), the IgK signal peptide (IgK) in frame with the gE2 ectodomain (gE2), the full-length gD (gD) provided with its transmembrane domain (TM) together with the cytoplasmic tail (ct), and the growth hormone polyadenylation signal (pA). (B) Chimeric peptide sequence (IgK-gE2/gD-TM) and predicted amino acid product. Italic ocher letters indicate the signal peptide, and the underlined black letters indicate the linker sequence connecting the signal peptide and the gE2 ectodomain (in red). gE2 is followed by the gD ectodomain in black and its transmembrane domain in blue, containing the highly hydrophobic peptide (underlined) and the cytoplasmic tail (in black).