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. 2009 Sep 30;16(11):1675–1686. doi: 10.1128/CVI.00224-09

FIG. 4.

FIG. 4.

(A) Representative fluorescent microscopic images of plaques formed by viable reconstituted recombinant BoHV-4-A-CMV-IgK-gE2gD-TM after DNA electroporation into BEK cells or in BEK cells expressing cre recombinase. Magnification, ×10. (B) Replication kinetics of BoHV-4-A-CMV-IgK-gE2gD-TM growth on cre-expressing cells, compared with those of the parental BoHV-4-A-CMV-IgK-gE2gD-TM still containing the BAC cassette and the BoHV-4-A isolate. The data presented are the means ± standard errors of triplicate measurements (P > 0.05 for all time points as measured by Student's t test). (C) Western immunoblotting of BoHV-4-A-CMV-IgK-gE2gD-TM-infected cell extracts. Uninfected BEK cell extract was used as a negative control.