FIG. 3.

Sterol synthesis is altered in Sre1 pathway mutants. Cells of the indicated strains were grown in YES medium at 21% or at 1% oxygen for 3 h at 30°C. Total sterols were extracted from each sample and analyzed by gas chromatography. Relative amounts of ergosterol were determined by normalizing data to an internal cholesterol standard. The experiment was repeated three times, and error bars indicate standard deviations. * and ** indicate that the difference is statistically significant compared to the wild type under normoxic and low-oxygen conditions, respectively (P < 0.05).