FIG. 2.

CaMss11 functions as an activator in hyphal development. (A) Overexpression of MSS11 promotes filamentous growth in C. albicans. Strain CAI4 (wild type) carrying pBA1 or pBA1-MSS11 was grown on Lee's plates at 25°C for 10 days. (B) CaMss11 exhibits strong transcriptional activity in S. cerevisiae. For the β-galactosidase assay, strain EGY48 carrying pGilda-CaMSS11 was transformed with or without vector pJG4-5. Three corresponding transformants were tested for the level of reporter gene expression using X-Gal. pSH17-4 was a positive control plasmid for LexA-AD fusion protein. pRFHM1 was a negative control plasmid for expression of a LexA-human lamin C fusion protein (43). The results are representative of three independent experiments.