FIG. 9.

Effects of Mss11 and Flo8 on hyphal growth under embedded condition. (A) C. albicans mss11/mss11 flo8/flo8 cells are unable to form hyphae in response to serum. Strains were streaked on the solid serum-containing medium and incubated at 37°C for 5 days or grown in liquid medium (YPD plus 10% serum) at 37°C for 3 h. (B and C) Deletion of MSS11 inhibits filaments formation under microaerophilic condition. Cells were plated with molten YPS agar and grown at 37°C for 2 days (B) or at 24°C for 3 days (C). Colony and cell morphologies of the wild-type strain (CAF2-1), the mss11/mss11 mutant (CSL3), the flo8/flo8 mutant (CCF3), and the mss11/mss11 flo8/flo8 double mutant (CSL5) are shown.