TABLE 4.
Haplotype and hybridization resultsa
| Fragment | Strain | Sequence type | No. of blot bands | Description |
|---|---|---|---|---|
| DbYER090 | Y866 | Polymorphic | 2 | |
| Y869 | Polymorphic | 2 | ||
| Y900 | Monomorphic | 1 | ||
| Y912 | Monomorphic | 2 | Gene conversion | |
| DbYDR513 | Y866 | Polymorphic | 2 | |
| Y869 | Polymorphic | 2 | ||
| Y900 | Polymorphic | 1 | ||
| Y912 | Polymorphic | 2 | ||
| DbYLR084 | Y866 | Monomorphic | 2 | Gene conversion |
| Y869 | Polymorphic | 3 | Multiple copies | |
| Y900 | Polymorphic | 1 | ||
| Y912 | Polymorphic | 2 | ||
| DbYDL040 | Y866 | Monomorphic | 2 | Gene conversion |
| Y869 | Polymorphic | 4 | Multiple copies | |
| Y900 | Polymorphic | 2 | ||
| Y912 | Monomorphic | 3 | Multiple copies/gene conversion | |
| HAD1 | Y866 | Polymorphic | 3 | Multiple copies |
| Y869 | Polymorphic | 1 | ||
| Y900 | Polymorphic | 2 | ||
| Y912 | Polymorphic | 2 |
a
Comparison between the sequence variation within a strain and the number of bands that hybridized to the respective probe by Southern blotting. The sequence analysis is based on the cloned PCR fragments. If only one sequence/haplotype was found in a strain, the locus was designated monomorphic. If there were more than two haplotypes, the locus was designated polymorphic. The blot band signals are derived from data shown in Fig. 4. The Southern blot was made from a PFGE gel, which separates chromosomes.